Transient expression assays using exonuclease deletion constructs spanning the 5′-flanking region of c-FLIP promoter element identified –121/+242 sequence with maximal constitutive reporter activity in both androgen-responsive LNCaP and androgen-independent prostate cancer cells PC-3 and DU145 cells (Fig. 1A–1C). This evidence concerns the gene CFLAR and prostate cancer.