We chose 33 cases of HER-2 positive breast cancer frozen tissue (18 cases with Lumina B like, 15 cases with HER2-positive type) for sequencing of 58 exons of the mTOR gene (next generation sequencing, Ion Torrent), nevertheless mutations that could cause an amino acid change were not found (data not shown). The gene discussed is ERBB2; the disease is breast carcinoma.