Oil red O staining revealed extensive accumulation of lipid droplets in the livers of Mrps34mut/mut mice that was significantly exacerbated with age (Fig. 6C and 6D) and correlated with increased levels of alanine aminotransferase (ALT) in the serum of these mice (Fig. 6E and 6F), which is a marker of liver dysfunction, commonly associated with liver steatosis [20]. This evidence concerns the gene GPT and Decreased liver function.