In order to analyze whether the Chagas disease patients CD8+ T cells recognize the TcCA-2-derived peptides in the context of the HLA-A*02:01+ molecule, peptide-pulsed K562-A2 cells were used as APC in a multiplex immunoassay for detection of IFNγ secretion (Table 2). The gene discussed is CD8A; the disease is Chagas disease.