The present study confirmed that IL-1β and TNF-α were induced by ischemia; however, IL-1β was downregulated in the serum of the GLGZD-2 group following treatment (P<0.05) and TNF-α was downregulated in the serum of the GLGZD-1 group following treatment (P<0.05). Here, IL1B is linked to ischemia.