No significant differences between 3-plex RT-qPCR assay and single RT-qPCR assay were observed in the PML-RARa/ABL NCN, MRD values and assay sensitivity for patients with APL after induction and consolidation (P > 0.05) (S2A-S2C Fig). The gene discussed is ABL1; the disease is acute promyelocytic leukemia.