Quantitative results of the 3-plex RT-qPCR were highly correlated with the results from single RT-qPCR and showed similar assay sensitivity for most of the PML-RARa positive APL samples at diagnosis and all samples during follow-up, except for one PML-RARa bcr2 case at diagnosis with breakpoint at 1579. This evidence concerns the gene RARA and acute promyelocytic leukemia.