Previous studies also found that the hypermethylated tumor-suppressor genes MlH1, TIMP3, p15 and p16 were not able to be activated by TSA alone in tumour cells; however, when a low dose of 5-Aza-CdR for slight demethylation was added, TSA treatment resulted in the restoration of the expression of all genes stated above (42). This evidence concerns the gene TIMP3 and neoplasm.