Co-IP analysis results was used to further confirmed the interaction between AFP and PTEN in the clinical specimens as observed in the cancerous HCC patients with AFP(+) tissues, and immunoprecipitation of the putative AFP-PTEN protein complex with anti-AFP antibody led to the obvious detection of an AFP band (Figure 1C), the finding that was consistent with our previous report in hematoma cell lines[10]. The gene discussed is PTEN; the disease is hepatocellular carcinoma.