TNF and hydrops fetalis: To validate the degree of hepatic inflammation in WT and Ldlr−/− mice fed a chow, HF, and HFC diet, we performed CD68 immunostaining and measured the expression of the proinflammatory genes Cd68, Cd11b, Tnfa, and Mcp in livers of WT and Ldlr−/− mice fed a chow, HF, and HFC diet.