AR and osteosarcoma: Sonneveld et al. (2005) established an androgen receptor gene reporter cell line named AR CALUX derived from human osteosarcoma cell line U2-OS using co-transfection of reporter plasmid 3xHRE-TATA-Luc and expression plasmid pSG5-neo-AR and they tested induction by DHT, maintenance of responsiveness and stability.