In addition, we examined the concentrations of IP-10 in equal amounts of protein (22.5 μl) obtained from the TY09 cells after exposure to the sera from the patients with NMO and RRMS as well as the healthy controls using another specified ELISA kit in order to confirm the reproducibility of the results obtained with the multiplexed fluorescent bead-based ELISA assay. This evidence concerns the gene CXCL10 and neuromyelitis optica.