Assays were repeated three times and representative pictures are shown in Fig. 3C. Quantification of the cell migration assay is shown in Fig. 3D. For invasion assays, we used hepatocyte growth factor (HGF) to induce migration, as it has been shown to stimulate the invasive potential of melanoma cells (McGill et al., 2006). The gene discussed is HGF; the disease is melanoma.