We first tested melanoma cells in culture, characterizing A375P (homozygous BRAFV600E), Mel624 (heterozygous BRAFV600E), and MeWo (homozygous BRAF WT) cell lines for BRAF protein expression (Pan-RAF and mutant BRAFV600E) on western blotting and immunofluorescence (S3 Fig). This evidence concerns the gene BRAF and melanoma.