PTEN and posterior cortical atrophy: As aforementioned, prostate gland is mainly a solid fibromuscular organ with a low permeability to fixative; therefore, unless special attention is paid, suboptimal tissue fixation is a common issue for prostatectomy specimen, which subsequently could affect the tissue antigen preservation and might at least partly explain the inconsistent results in some of the immunohistochemical studies of PTEN in PCa.