AGO2 and infection: Local infection foci of suppressor-deficient TuMV-AS9-GFP were readily visible at 7 days post inoculation (dpi) in ago2–1 mutant plants (Fig. 1B and Table 1), and infection efficiency was not significantly different than that of the dcl2–1 dcl3–1 dcl4–2 triple mutant, which served as the hypersusceptible, silencing-deficient control (Fig. 1B) [23].