Combining ago1–27 with ago10–5, or with ago2–1 and ago10–5 in a triple mutant, had no effects on local TuMV-AS9-GFP infection foci (Fig. 2A panel II) or accumulation in cauline leaves beyond those measured in the single ago2 or double ago2 ago10 mutants (Fig. 2B panels I and II, and Table 3). This evidence concerns the gene AGO2 and infection.