Local TuMV-AS9-GFP infection foci were observed in inoculated rosette leaves, and virus was detected in noninoculated cauline leaves, from ago1–27 ago2–1 double mutant plants, but ago1–27 had no enhancing or suppressing effects when combined with ago2–1 (panel II in Fig. 2A and 2B, Table 3). This evidence concerns the gene AGO1 and infection.