TWIST1 and neoplasm: TWIST1 and TWIST2 immunohistochemistry on 2 cohorts: cohort 1 (multi-punch TMA) + promoter methylation. Cohort 2: TMA from pre-operative biopsies (prognostic effects). Immunohistochemistry for both markers and promoter methylation in six cell lines. LCM in one tumor-budding high and one tumor-budding low case