Using 501Mel melanoma cell lines stably expressing an N-terminal FLAG-HA epitope-tagged MITF (F-H-MITF) to levels comparable to that of endogenous MITF (Figure 1A, line J), we performed tandem affinity purification of soluble nuclear-and chromatin-associated fractions (Drané et al., 2010). Here, MITF is linked to melanoma.