To determine the prevalence and clinical significance of miR-21 and its direct targets PTPN14 and PTEN in ICC, we assessed the expression of miR-21, PTPN14 and PTEN by miRNA in situ hybridization and IHC staining in tumor tissue samples from 57 ICC patients who received curative resection. This evidence concerns the gene PTPN14 and intrahepatic cholangiocarcinoma.