The gene expression analysis of muscle fiber type markers indicated a shift in muscle fiber type composition, with a significant increase in fast-oxidative type 2A fibers (MYH2 mRNA) and a similar trend for fast-glycolytic type 2X fibers (MYH1 mRNA) in muscle of prediabetic and T2D individuals compared to the healthy lean subjects (MYH2 mRNA: obese: 1.1-fold (p>0.1), preT2D: 1.8-fold (p<0.05), T2D: 1.6-fold (p = 0.07) over lean and MYH1 mRNA: obese: 2.2-fold (p>0.1), preT2D: 2.7-fold (p = 0.07), T2D: 2.5-fold (p>0.1) over lean). The gene discussed is MYH2; the disease is type 2 diabetes mellitus.