To evaluate the expression of ABCD3 during prostate cancer progression, immunohistochemistry was conducted in Caucasian American ABCD3 expression in 206 cores from 114 Caucasian patients, including normal tissue (20 patients), low Gleason tumors (87 patients), and high Gleason tumors (81 patients). ABCD3 expression was detected using immunohistochemical staining with an anti-ABCD3, a polyclonal antibody; the intensity of brown staining corresponds to the level of ABCD3 expression. The gene discussed is ABCD3; the disease is prostate cancer.