IL10, WNT5A, OPAL1, CCND2, and CASP8AP2 normalized ΔCq presented the most marked differences with respect to the controls; WNT5A was the gene with the best sensitivity/specificity trade-off and therefore the best potential B-ALL diagnosis biomarker evaluated in this study. The gene discussed is WNT5A; the disease is acute lymphoblastic leukemia.