The specificity of anti-HPIP antibody was confirmed by immunohistochemical staining of CRC tissues incubated with anti-HPIP preincubated with its antigen (Supplementary Fig. 1A) and immunoblotting of lysates from HCT-8 and SW480 CRC cells infected with HPIP shRNA (Supplementary Fig. 1B). Here, PBXIP1 is linked to colorectal carcinoma.