The two pancreatic cancer cell lines were transfected with siRNA targeting HIF-1α and cultured under hypoxia (1% O2) for 12 hrs (defined as Hsi, the same below), and the two cell lines only cultured under hypoxia (1% O2) for 12 hrs were used as the control sample (defined as HC, the same below), as shown in Fig. 2C and D. Here, HIF1A is linked to familial pancreatic carcinoma.