Immunofluorescence staining of CWR22Rν1 & C4-2 cells showed decrease nuclear localization of AR in MEM treated cells as compared to control (Fig. 5B).The CWR22Rν1 and C4-2 prostate cancer cells exhibit high protein levels of intracellular PSA, as evidenced by a 34-kDa PSA band (Fig. 5A). The gene discussed is AR; the disease is prostate carcinoma.