Herein, we demonstrate that miR-432 simultaneously suppresses three important component of the wnt pathway, i.e. LRP6, TRIM29, and Pygo2 expression by directly targeting their 3′UTRs and that β-catenin nuclear accumulation could be robustly repressed by miR-432 overexpression in HCC. The gene discussed is PYGO2; the disease is hepatocellular carcinoma.