The principal mechanisms of MLN4924 cytotoxicity in cancer cells appear to involve blocking NF-κB signaling (via stabilization of IκBα, and the retention of the NF-κB transcription factor in the cytoplasm) or leading to unlicensed DNA replication (via stabilization Cdt-1), ultimately leading to apoptotic cells death. The gene discussed is NFKBIA; the disease is cancer.