WT1 and acute myeloid leukemia: To ensure that the phenomenon of hypoxia-mediated hypomethylation of the WT1 Intron 1 CpG island resulting in upregulation of both WT1 lncRNA and WT1 mRNA is not an artifact of cell culture, we evaluated Intron 1 methylation status and expression of both WT1 mRNA and WT1 lncRNA in a panel of primary acute myeloid leukemia (AML) samples.