HNF1A and pancreatic adenocarcinoma: Using quantitative RT-PCR (qRT-PCR) and Western blot analyses, we showed that HNF1A was expressed in three well-characterized pancreatic carcinoma cell lines derived either from the primary pancreatic adenocarcinoma tumors (BxPC-3) or metastatic sites (AsPC-1, Colo357) with the highest level detected in AsPC-1 (Fig. 1A).