To examine whether α-catulin and E-cadherin have synergistic effects melanoma cells were transfected with a NF-ĸB reporter gene (5x NF-ĸB-Luc) plus sh-catu (downregulation) or myc-α-catulin (ectopic expression) together with or without si-E-cadherin RNA (Fig. 1F). Here, MYC is linked to melanoma.