Immunohistochemistry for HIF-1α expresssion showed few hepatocytes expressing HIF in saline groups, and cells expressing the HIF protein in DEN groups were mostly residing in the portal area, and in and around hepatocellular- and cholangioma lesions, coinciding with CK19 and Epcam immunopositive regions. The gene discussed is HIF1A; the disease is bile duct adenoma.