Protein expression levels of c-fos (a biochemical marker of anxiety), the enzymes of antioxidant defense including glyoxalase (GLO)-1, glutathione reductase (GSR)-1, manganese (Mn) superoxide dismutase (SOD) and copper-zinc (Cu/Zn) SOD, inflammatory markers and a regulator of G-protein signaling protein (RGS)-2 also were examined. This evidence concerns the gene GLO1 and Anxiety.