At a dose of 40 μg AmB in AP, there was no prophylactic benefit seen using any of the following parameters; histology, CFU, quantitative PCR for Aspergillus ribosomal 28S rRNA, and quantitative RT-PCR of mRNA for analysis of TNF-α, IFN-γ, MIP-1β, IL-10 and iNOS in mouse lung (n = 6/group). This evidence concerns the gene TNF and alkaline phosphatase measurement.