Accordingly, Western blot analysis (Fig. 8a, b) revealed that the 320-kDa fragment, representing the secreted form of Reelin[32], was significantly decreased in KA-treated PRNCs compared to control, whereas the full-length Reelin protein (400 kDa) in KA-treated PRNCs was significantly increased (p < 0.05), indicating that proteolytic processing of Reelin was decreased in this acute KA neurotoxicity paradigm that mimics the early stage of epilepsy. This evidence concerns the gene RELN and epilepsy.