CD4 and breast cancer: However, bias estimation of manual bead based methods showed underestimation of CD4 counts at <350 cells/μl of-35.2 cells/μl (limits of agreement-164.9,+ 94.6) and -0.4 cells/μl (limits of agreement-126, +125.2) for BC Cytospheres and Dynal Dynabeads respectively [18].