When plasma from NSCLC patients were pretreated with DNase or RNase and then incubated with the normal PBMCs, as shown in Figure 2A and B, we found that pretreatment of plasma with DNase and RNase could significantly abrogated the induction of IL-8, IFN-α and TNF-α in a dose dependent manner (P < 0.05), suggesting that nucleic acid was crucial for stimulatory activity of NSCLC plasma. Here, TNF is linked to non-small cell lung carcinoma.