In past studies, the Wingless-type (Wnt) signaling, osteoclasts activating factors, transcription factor Runx2 [6], receptor activator of nuclear factor-κB (RANK)/RANK ligand (RANKL)/osteoprotegerin(OPG) system, IL-3, IL-7, and tumor necrosis factor (TNF)-α all involved in the inhibition of osteoblasts in MBD. This evidence concerns the gene TNF and Marchiafava-Bignami disease.