Analysis of urea-soluble fractions from prefrontal cortex also revealed that a 35-kDa lower molecular weight species of TDP-43 was clearly present in four of six ALS-FTLD cases, absent in controls, and was also labeled with Met85-TDP-35 antibody, again suggesting that this pathological form of TDP-43 in the brain is generated by alternate translation starting at Met85. The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.