As has been reported for other N-terminally truncated 35-kDa species of TDP-43 [9, 29], Met85-TDP-35 had reduced solubility, a cytoplasmic localization and formed aggregates when expressed in human neuroblastoma cells, all of which are predominant features of pathological TDP-43 in ALS and FTLD [1, 15]. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.