Different from previous studies which showed a differentially activation of p38, JNK and Erk in distinct cancer cells [9,10,15], we demonstrated that PL induced prominent activation of all the three major MAPKs signaling pathways including p38, JNK and Erk in HCC cells, supporting that MAPKs were major ROS-stress responsive signaling pathways upon PL treatment. This evidence concerns the gene MAPK8 and cancer.