In our present series, NOTCH1 mutation was confirmed to be strongly associated with trisomy 12, IGHV4–39 gene usage and with shorter TTFT in univariate analysis, whereas no difference in NOTCH1 mutation prevalence was observed comparing cMBL, with CLL or Rai 0 patients (data not shown). The gene discussed is NOTCH1; the disease is B-cell chronic lymphocytic leukemia.