IFNG and tuberculosis: The number of total INF-γ+, IL-2+ IFN-γ+ and IL-2- IFN-γ+ was always significantly lower in healthy subjects in comparison with active tuberculosis, past tuberculosis and LTBI, as well as the SFC of total IL-2+ and IL-2+ IFN-γ-secreting cells of healthy EliSpot-IGRA-negative controls in comparison with tuberculosis patients.