CD4 and brain neoplasm: In the brain tumor region, the CD3+CD4+ TIL population showed no significant increase induced by FUS exposure alone when compared to sham group (p = 0.016), but was significantly increased by either IL-12 alone and combined FUS + IL-12 (22.16 ± 7.75% and 20.83 ± 5.28% with p = 0.002 and <0.001, respectively) (Figure 4(A)).