We performed immunohistochemistry using the Ventana BRAF V600E (VE1) mouse monoclonal primary antibody on Ventana Benchmark IHC automated strainers in combination with the OptiView DAB IHC detection kit in CRC, PTC and melanoma samples, and the results show highly sensitivity (100%) and specificity (99%). The gene discussed is BRAF; the disease is colorectal carcinoma.