DACH1 and cancer: In contrast to DACH1, silencing of PCP4 in H295R cells decreased CYP11B2 and aldosterone production, whereas in APAs, the mRNA levels of PCP4 positively correlated with those of CYP11B2. 10 Furthermore, the change from purely nuclear to cytoplasmic staining for DACH1 in some of the clusters resembles that reported in the ovary, at the onset of cancer.25