We evaluated 22-kHz ultrasonic vocalizations (emitted in aversive contexts [15]), open-field behavior (to evaluate exploratory/motor and anxiety parameters [16, 17]), and plasma TNF-α, corticosterone (an indicator of stress and HPA axis activity [18]), and levels of brain-derived neurotrophic factor (BDNF, as LPS and zinc may interfere with BDNF expression [19, 20]). This evidence concerns the gene BDNF and Anxiety.