Expression levels of endogenousCXCR4 and AKT (phosphorylated AKTS473 and total AKT) proteins varied among the four human lung cancer (H1299, HCC827, H460, and A549) cell lines tested with H1299 cell line showing the highest expression level of the two proteins (Fig. 1A). The gene discussed is AKT1; the disease is lung carcinoma.