We then combined a cmNTS injection of an anorectic dose of L-leucine (105 pmol/side in LF- fed mice, 1.05nmol/side in HF-fed mice) or vehicle with a subthreshold dose of ip CCK (1 μg/kg in controls, and 2 μg/kg in HF-fed mice) or vehicle. Here, CCK is linked to hydrops fetalis.