Moreover, we measured by reverse phase protein arrays that in tumors the extent of TEM infiltration was significantly and linearly correlated with PLGF content (Fig. 7A) thus highlighting that Tie-2 and VEGFR-1 axes, as well as their cognate angiogenic TEM ligands Ang-2 and PlGF represent attractive therapeutic targets in breast cancer. This evidence concerns the gene PGF and breast cancer.