For examples, the GRK4 A486V mutant increased the obesity risk 1.75-fold more when the obesity index was used (P-trend = 0.015), however, the GRK4 A142V mutant decreased the obesity risk by 0.7-fold (P-trend = 0.05) when the WC was used (P-trend = 0.058). This evidence concerns the gene GRK4 and obesity due to melanocortin 4 receptor deficiency.