Hence, the current study has been designed to measure the plasma concentration of CTRP1 in patients with NAFLD, T2DM and NAFLD with T2DM (NAFLD+T2DM) compared with healthy subjects and also to study its association with the metabolic and hepatic profile and also liver stiffness. The gene discussed is C1QTNF1; the disease is metabolic dysfunction-associated steatotic liver disease.