We observed by flow cytometry, that intracellular secretion of IL-10 and TGF-β was higher in HBV-HCC Tregs than non-HBVHCC (IL-10; P = 0.01; and TGF-β; P = 0.04, Figures 4B,C). This evidence concerns the gene TGFB1 and hepatocellular carcinoma.